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antibodies against c myc sc 40  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology antibodies against c myc sc 40
    Antibodies Against C Myc Sc 40, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+myc+antibody/c-Myc/pmc13652265-188-0-10
    Average 96 stars, based on 3157 article reviews
    antibodies against c myc sc 40 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Supporting Information Discovery and Characterization of a Highly Potent and Selective Aminopyrazoline-Based in vivo Probe (BAY-598) for the Protein Lysine Methyltransferase SMYD2
    Article Snippet: Cells were fixed for 10 min with 4% paraformaldehyde (Santa Cruz), permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) and blocked with 1.0% bovine serum albumin (Sigma-Aldrich) all in PBS buffer. .. Staining was done using AHNAK antibody (1:200), SY46 antibody (1:200) for detection of methylation and c-myc antibody (Santa Cruz Biotechnology, #sc-40, 1:500) (for detection of the c-Myc tag of the SMYD2 protein) combined with secondary antibodies Alexa Fluor 488 goat anti-rabbit (Thermo Fisher Scientific, #A11008, 1:500) and Alexa Fluor 647 goat anti-mouse (Thermo Fisher Scientific, # A-21236, 1:500). .. F-actin was stained with Alexa Fluor 555 Phalloidin (Thermo Fisher Scientific, #A34055, 1:30 in PBS).

    Methylation:

    Article Title: Supporting Information Discovery and Characterization of a Highly Potent and Selective Aminopyrazoline-Based in vivo Probe (BAY-598) for the Protein Lysine Methyltransferase SMYD2
    Article Snippet: Cells were fixed for 10 min with 4% paraformaldehyde (Santa Cruz), permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) and blocked with 1.0% bovine serum albumin (Sigma-Aldrich) all in PBS buffer. .. Staining was done using AHNAK antibody (1:200), SY46 antibody (1:200) for detection of methylation and c-myc antibody (Santa Cruz Biotechnology, #sc-40, 1:500) (for detection of the c-Myc tag of the SMYD2 protein) combined with secondary antibodies Alexa Fluor 488 goat anti-rabbit (Thermo Fisher Scientific, #A11008, 1:500) and Alexa Fluor 647 goat anti-mouse (Thermo Fisher Scientific, # A-21236, 1:500). .. F-actin was stained with Alexa Fluor 555 Phalloidin (Thermo Fisher Scientific, #A34055, 1:30 in PBS).

    Immunoprecipitation:

    Article Title: Promoter-proximal transcription–translation coupling controls early transcription in Escherichia coli
    Article Snippet: .. DNA-NusG complexes were immunoprecipitated using 15 μl c-Myc antibody (Santa Cruz Biotech), and all other experimental steps were performed as described earlier. ..

    Western Blot:

    Article Title: Spt5 orchestrates cryptic transcript suppression and transcriptional directionality
    Article Snippet: .. Antibodies used in this research Antibody cat no. Used experiments and concentrations anti-RNA Polymerase II RPB1 antibody (8wg16) (664906, Biolegend) Western blot (1:5000), ChIP (1:66) anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721, Abcam) Western blot (1:10000) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab5095, Abcam) Western blot (1:10000), ChIP (1:100) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131, Abcam) ChIP (1:100) mouse anti-Flag antibody (F1804, Abcam) Western blot (1:3000), Co-IP (1:500) anti-HA hybrid antibody Purified from rabbit Western blot (1:3000), Co-IP (1:500) c-Myc antibody (9E10, Santa Cruz) Western blot (1:3000) p-Spt5p antibody Purified from rabbit Western blot (1:1000) Yeast H3 antibody Purified from rabbit Western blot (1:5000), ChIP (1:50) anti-H3K36me3 Purified from rabbit ChIP (1:100) anti-H3K79me3 Purified from rabbit ChIP (1:100) anti-histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915, Abcam) ChIP (1:100) Histone H4ac (pan-acetyl) antibody (39244, Active Motif) ChIP (1:100) β-actin antibody (sc-47778, Santa Cruz) Western blot (1:1000) V5-Tag antibody (MCA1360, Biorad) Western blot (1:3000) Goat anti-mouse IgG (H+L) (115-035-003, Jackson ImmunoResearch) Western blot (1:10000) Goat anti-rabbit IgG (H+L) (111-035-003, Jackson ImmunoResearch) Western blot (1:10000) ..

    Chromatin Immunoprecipitation:

    Article Title: Spt5 orchestrates cryptic transcript suppression and transcriptional directionality
    Article Snippet: .. Antibodies used in this research Antibody cat no. Used experiments and concentrations anti-RNA Polymerase II RPB1 antibody (8wg16) (664906, Biolegend) Western blot (1:5000), ChIP (1:66) anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721, Abcam) Western blot (1:10000) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab5095, Abcam) Western blot (1:10000), ChIP (1:100) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131, Abcam) ChIP (1:100) mouse anti-Flag antibody (F1804, Abcam) Western blot (1:3000), Co-IP (1:500) anti-HA hybrid antibody Purified from rabbit Western blot (1:3000), Co-IP (1:500) c-Myc antibody (9E10, Santa Cruz) Western blot (1:3000) p-Spt5p antibody Purified from rabbit Western blot (1:1000) Yeast H3 antibody Purified from rabbit Western blot (1:5000), ChIP (1:50) anti-H3K36me3 Purified from rabbit ChIP (1:100) anti-H3K79me3 Purified from rabbit ChIP (1:100) anti-histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915, Abcam) ChIP (1:100) Histone H4ac (pan-acetyl) antibody (39244, Active Motif) ChIP (1:100) β-actin antibody (sc-47778, Santa Cruz) Western blot (1:1000) V5-Tag antibody (MCA1360, Biorad) Western blot (1:3000) Goat anti-mouse IgG (H+L) (115-035-003, Jackson ImmunoResearch) Western blot (1:10000) Goat anti-rabbit IgG (H+L) (111-035-003, Jackson ImmunoResearch) Western blot (1:10000) ..

    Co-Immunoprecipitation Assay:

    Article Title: Spt5 orchestrates cryptic transcript suppression and transcriptional directionality
    Article Snippet: .. Antibodies used in this research Antibody cat no. Used experiments and concentrations anti-RNA Polymerase II RPB1 antibody (8wg16) (664906, Biolegend) Western blot (1:5000), ChIP (1:66) anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721, Abcam) Western blot (1:10000) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab5095, Abcam) Western blot (1:10000), ChIP (1:100) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131, Abcam) ChIP (1:100) mouse anti-Flag antibody (F1804, Abcam) Western blot (1:3000), Co-IP (1:500) anti-HA hybrid antibody Purified from rabbit Western blot (1:3000), Co-IP (1:500) c-Myc antibody (9E10, Santa Cruz) Western blot (1:3000) p-Spt5p antibody Purified from rabbit Western blot (1:1000) Yeast H3 antibody Purified from rabbit Western blot (1:5000), ChIP (1:50) anti-H3K36me3 Purified from rabbit ChIP (1:100) anti-H3K79me3 Purified from rabbit ChIP (1:100) anti-histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915, Abcam) ChIP (1:100) Histone H4ac (pan-acetyl) antibody (39244, Active Motif) ChIP (1:100) β-actin antibody (sc-47778, Santa Cruz) Western blot (1:1000) V5-Tag antibody (MCA1360, Biorad) Western blot (1:3000) Goat anti-mouse IgG (H+L) (115-035-003, Jackson ImmunoResearch) Western blot (1:10000) Goat anti-rabbit IgG (H+L) (111-035-003, Jackson ImmunoResearch) Western blot (1:10000) ..

    Purification:

    Article Title: Spt5 orchestrates cryptic transcript suppression and transcriptional directionality
    Article Snippet: .. Antibodies used in this research Antibody cat no. Used experiments and concentrations anti-RNA Polymerase II RPB1 antibody (8wg16) (664906, Biolegend) Western blot (1:5000), ChIP (1:66) anti-RNA polymerase II CTD repeat YSPTSPS antibody - ChIP Grade (ab26721, Abcam) Western blot (1:10000) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody (ab5095, Abcam) Western blot (1:10000), ChIP (1:100) anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131, Abcam) ChIP (1:100) mouse anti-Flag antibody (F1804, Abcam) Western blot (1:3000), Co-IP (1:500) anti-HA hybrid antibody Purified from rabbit Western blot (1:3000), Co-IP (1:500) c-Myc antibody (9E10, Santa Cruz) Western blot (1:3000) p-Spt5p antibody Purified from rabbit Western blot (1:1000) Yeast H3 antibody Purified from rabbit Western blot (1:5000), ChIP (1:50) anti-H3K36me3 Purified from rabbit ChIP (1:100) anti-H3K79me3 Purified from rabbit ChIP (1:100) anti-histone H3 (acetyl K9 + K14 + K18 + K23 + K27) antibody (ab47915, Abcam) ChIP (1:100) Histone H4ac (pan-acetyl) antibody (39244, Active Motif) ChIP (1:100) β-actin antibody (sc-47778, Santa Cruz) Western blot (1:1000) V5-Tag antibody (MCA1360, Biorad) Western blot (1:3000) Goat anti-mouse IgG (H+L) (115-035-003, Jackson ImmunoResearch) Western blot (1:10000) Goat anti-rabbit IgG (H+L) (111-035-003, Jackson ImmunoResearch) Western blot (1:10000) ..



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